Inhibition of SARS-CoV replication by siRNA.
Identifieur interne : 004784 ( Main/Exploration ); précédent : 004783; suivant : 004785Inhibition of SARS-CoV replication by siRNA.
Auteurs : Chang-Jer Wu [République populaire de Chine] ; Hui-Wen Huang ; Chiu-Yi Liu ; Cheng-Fong Hong ; Yi-Lin ChanSource :
- Antiviral research [ 0166-3542 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Cellules Vero, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (métabolisme), Humains, Interférence par ARN, Petit ARN interférent (métabolisme), Petit ARN interférent (pharmacologie), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Régions 3' non traduites (génétique), Régions 3' non traduites (métabolisme), Réplication virale (), Syndrome respiratoire aigu sévère (virologie), Virus du SRAS (), Virus du SRAS (physiologie).
- MESH :
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Régions 3' non traduites.
- métabolisme : Glycoprotéines membranaires, Petit ARN interférent, Protéines de l'enveloppe virale, Régions 3' non traduites.
- pharmacologie : Petit ARN interférent.
- physiologie : Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Cellules Vero, Glycoprotéine de spicule des coronavirus, Humains, Interférence par ARN, Réplication virale, Virus du SRAS.
English descriptors
- KwdEn :
- 3' Untranslated Regions (genetics), 3' Untranslated Regions (metabolism), Animals, Chlorocebus aethiops, Humans, Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), RNA Interference, RNA, Small Interfering (metabolism), RNA, Small Interfering (pharmacology), SARS Virus (drug effects), SARS Virus (physiology), Severe Acute Respiratory Syndrome (virology), Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism), Virus Replication (drug effects).
- MESH :
- chemical , genetics : 3' Untranslated Regions, Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , metabolism : 3' Untranslated Regions, Membrane Glycoproteins, RNA, Small Interfering, Viral Envelope Proteins.
- chemical , pharmacology : RNA, Small Interfering.
- drug effects : SARS Virus, Virus Replication.
- physiology : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Chlorocebus aethiops, Humans, RNA Interference, Spike Glycoprotein, Coronavirus, Vero Cells.
Abstract
Serious outbreaks of severe acute respiratory syndrome (SARS), caused by the newly discovered coronavirus SARS-CoV, occurred between late 2002 and early 2003 and there is an urgent need for effective antiviral agents. RNA interference in animals and post-transcriptional gene silencing plants is mediated by small double-stranded RNA molecules named small interfering RNA (siRNA). Recently, siRNA-induced RNA interference(RNAi) may provide a new approach to therapy for pathogenic viruses, e.g. HIV and HCV. In this study, the silencing potential of seven synthetic siRNAs against SARS-CoV leader, TRS, 3'-UTR and Spike coding sequence have been applied to explore the possibility for prevention of SARS-CoV infection. We demonstrate that siRNAs directed against Spike sequences and the 3'-UTR can inhibit the replication of SARS-CoV in Vero-E6 cells, and holds out promise for the development of an effective antiviral agent against SARS-CoV.
DOI: 10.1016/j.antiviral.2004.09.005
PubMed: 15652970
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002936
- to stream PubMed, to step Curation: 002936
- to stream PubMed, to step Checkpoint: 002652
- to stream Ncbi, to step Merge: 000D31
- to stream Ncbi, to step Curation: 000D31
- to stream Ncbi, to step Checkpoint: 000D31
- to stream Main, to step Merge: 004A67
- to stream Main, to step Curation: 004784
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Inhibition of SARS-CoV replication by siRNA.</title>
<author><name sortKey="Wu, Chang Jer" sort="Wu, Chang Jer" uniqKey="Wu C" first="Chang-Jer" last="Wu">Chang-Jer Wu</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Food Science, National Taiwan Ocean University, Keelung, Pei Ning Road, Keelung 202, Taiwan, ROC. wuchangjer@yahoo.com.tw</nlm:affiliation>
<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
<wicri:regionArea>Department of Food Science, National Taiwan Ocean University, Keelung, Pei Ning Road, Keelung 202, Taiwan</wicri:regionArea>
<wicri:noRegion>Taiwan</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Huang, Hui Wen" sort="Huang, Hui Wen" uniqKey="Huang H" first="Hui-Wen" last="Huang">Hui-Wen Huang</name>
</author>
<author><name sortKey="Liu, Chiu Yi" sort="Liu, Chiu Yi" uniqKey="Liu C" first="Chiu-Yi" last="Liu">Chiu-Yi Liu</name>
</author>
<author><name sortKey="Hong, Cheng Fong" sort="Hong, Cheng Fong" uniqKey="Hong C" first="Cheng-Fong" last="Hong">Cheng-Fong Hong</name>
</author>
<author><name sortKey="Chan, Yi Lin" sort="Chan, Yi Lin" uniqKey="Chan Y" first="Yi-Lin" last="Chan">Yi-Lin Chan</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2005">2005</date>
<idno type="RBID">pubmed:15652970</idno>
<idno type="pmid">15652970</idno>
<idno type="doi">10.1016/j.antiviral.2004.09.005</idno>
<idno type="wicri:Area/PubMed/Corpus">002936</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002936</idno>
<idno type="wicri:Area/PubMed/Curation">002936</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002936</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002652</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002652</idno>
<idno type="wicri:Area/Ncbi/Merge">000D31</idno>
<idno type="wicri:Area/Ncbi/Curation">000D31</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000D31</idno>
<idno type="wicri:doubleKey">0166-3542:2005:Wu C:inhibition:of:sars</idno>
<idno type="wicri:Area/Main/Merge">004A67</idno>
<idno type="wicri:Area/Main/Curation">004784</idno>
<idno type="wicri:Area/Main/Exploration">004784</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Inhibition of SARS-CoV replication by siRNA.</title>
<author><name sortKey="Wu, Chang Jer" sort="Wu, Chang Jer" uniqKey="Wu C" first="Chang-Jer" last="Wu">Chang-Jer Wu</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Food Science, National Taiwan Ocean University, Keelung, Pei Ning Road, Keelung 202, Taiwan, ROC. wuchangjer@yahoo.com.tw</nlm:affiliation>
<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
<wicri:regionArea>Department of Food Science, National Taiwan Ocean University, Keelung, Pei Ning Road, Keelung 202, Taiwan</wicri:regionArea>
<wicri:noRegion>Taiwan</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Huang, Hui Wen" sort="Huang, Hui Wen" uniqKey="Huang H" first="Hui-Wen" last="Huang">Hui-Wen Huang</name>
</author>
<author><name sortKey="Liu, Chiu Yi" sort="Liu, Chiu Yi" uniqKey="Liu C" first="Chiu-Yi" last="Liu">Chiu-Yi Liu</name>
</author>
<author><name sortKey="Hong, Cheng Fong" sort="Hong, Cheng Fong" uniqKey="Hong C" first="Cheng-Fong" last="Hong">Cheng-Fong Hong</name>
</author>
<author><name sortKey="Chan, Yi Lin" sort="Chan, Yi Lin" uniqKey="Chan Y" first="Yi-Lin" last="Chan">Yi-Lin Chan</name>
</author>
</analytic>
<series><title level="j">Antiviral research</title>
<idno type="ISSN">0166-3542</idno>
<imprint><date when="2005" type="published">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>3' Untranslated Regions (genetics)</term>
<term>3' Untranslated Regions (metabolism)</term>
<term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Humans</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (metabolism)</term>
<term>RNA Interference</term>
<term>RNA, Small Interfering (metabolism)</term>
<term>RNA, Small Interfering (pharmacology)</term>
<term>SARS Virus (drug effects)</term>
<term>SARS Virus (physiology)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (metabolism)</term>
<term>Virus Replication (drug effects)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Cellules Vero</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (génétique)</term>
<term>Glycoprotéines membranaires (métabolisme)</term>
<term>Humains</term>
<term>Interférence par ARN</term>
<term>Petit ARN interférent (métabolisme)</term>
<term>Petit ARN interférent (pharmacologie)</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Régions 3' non traduites (génétique)</term>
<term>Régions 3' non traduites (métabolisme)</term>
<term>Réplication virale ()</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Virus du SRAS ()</term>
<term>Virus du SRAS (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>3' Untranslated Regions</term>
<term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>3' Untranslated Regions</term>
<term>Membrane Glycoproteins</term>
<term>RNA, Small Interfering</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>RNA, Small Interfering</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>SARS Virus</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Régions 3' non traduites</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Petit ARN interférent</term>
<term>Protéines de l'enveloppe virale</term>
<term>Régions 3' non traduites</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Petit ARN interférent</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Humans</term>
<term>RNA Interference</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules Vero</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Humains</term>
<term>Interférence par ARN</term>
<term>Réplication virale</term>
<term>Virus du SRAS</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Serious outbreaks of severe acute respiratory syndrome (SARS), caused by the newly discovered coronavirus SARS-CoV, occurred between late 2002 and early 2003 and there is an urgent need for effective antiviral agents. RNA interference in animals and post-transcriptional gene silencing plants is mediated by small double-stranded RNA molecules named small interfering RNA (siRNA). Recently, siRNA-induced RNA interference(RNAi) may provide a new approach to therapy for pathogenic viruses, e.g. HIV and HCV. In this study, the silencing potential of seven synthetic siRNAs against SARS-CoV leader, TRS, 3'-UTR and Spike coding sequence have been applied to explore the possibility for prevention of SARS-CoV infection. We demonstrate that siRNAs directed against Spike sequences and the 3'-UTR can inhibit the replication of SARS-CoV in Vero-E6 cells, and holds out promise for the development of an effective antiviral agent against SARS-CoV.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
</list>
<tree><noCountry><name sortKey="Chan, Yi Lin" sort="Chan, Yi Lin" uniqKey="Chan Y" first="Yi-Lin" last="Chan">Yi-Lin Chan</name>
<name sortKey="Hong, Cheng Fong" sort="Hong, Cheng Fong" uniqKey="Hong C" first="Cheng-Fong" last="Hong">Cheng-Fong Hong</name>
<name sortKey="Huang, Hui Wen" sort="Huang, Hui Wen" uniqKey="Huang H" first="Hui-Wen" last="Huang">Hui-Wen Huang</name>
<name sortKey="Liu, Chiu Yi" sort="Liu, Chiu Yi" uniqKey="Liu C" first="Chiu-Yi" last="Liu">Chiu-Yi Liu</name>
</noCountry>
<country name="République populaire de Chine"><noRegion><name sortKey="Wu, Chang Jer" sort="Wu, Chang Jer" uniqKey="Wu C" first="Chang-Jer" last="Wu">Chang-Jer Wu</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004784 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004784 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:15652970 |texte= Inhibition of SARS-CoV replication by siRNA. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:15652970" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
This area was generated with Dilib version V0.6.33. |